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AMPK alpha 1 (phospho Ser496) antibody

Anti-AMPK alpha 1 (phospho Ser496) antibody used in IHC (Paraffin sections) (IHC-P). GTX32345
Anti-AMPK alpha 1 (phospho Ser496) antibody used in Western Blot (WB). GTX32345
Anti-AMPK alpha 1 (phospho Ser496) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32345

Cat No. GTX32345

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($319)
See all AMPK alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of AMPK alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 1 , AMPK , AMPKa1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 (phospho Ser496) antibody used in IHC (Paraffin sections) (IHC-P). GTX32345

GTX32345 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32345 AMPK alpha 1 (phospho Ser496) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-AMPK alpha 1 (phospho Ser496) antibody used in Western Blot (WB). GTX32345

GTX32345 WB Image

WB analysis of UV-treated HUVEC (A), HeLa (B), SP2/0 (C), PC12 (D) whole cell lysates using GTX32345 AMPK alpha 1 (phospho Ser496) antibody.

Anti-AMPK alpha 1 (phospho Ser496) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32345

GTX32345 ICC/IF Image

ICC/IF analysis of formalin-fixed HUVEC cells using GTX32345 AMPK alpha 1 (phospho Ser496) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319