GeneTex
United States (US)

AMPK alpha 1 (phospho Ser496) antibody

GTX32345_20190131_IHC-P_19013101_320.jpg
GTX32345_20190131_WB_19013101_296.jpg
GTX32345_20190131_ICCIF_19013101_117.jpg

Cat No. GTX32345

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
See all AMPK alpha 1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

64 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of AMPK alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Kinase Amp-Activated Catalytic Subunit Alpha 1,Ampk,Ampka1,Prkaa1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32345_20190131_IHC-P_19013101_320.jpg

GTX32345 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32345 AMPK alpha 1 (phospho Ser496) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
GTX32345_20190131_WB_19013101_296.jpg

GTX32345 WB Image

WB analysis of UV-treated HUVEC (A), HeLa (B), SP2/0 (C), PC12 (D) whole cell lysates using GTX32345 AMPK alpha 1 (phospho Ser496) antibody.
GTX32345_20190131_ICCIF_19013101_117.jpg

GTX32345 ICC/IF Image

ICC/IF analysis of formalin-fixed HUVEC cells using GTX32345 AMPK alpha 1 (phospho Ser496) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Package List Price ($)
$ 319