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AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX03702
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Flow cytometry (FACS). GTX03702
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX03702
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX03702
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX03702
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX03702
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX03702
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX03702
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX03702

Cat. No. GTX03702

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat, Sheep, Pig
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF Assay dependent
IHC-P 1:100-1:500
FACS 1:100
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Chicken, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

10mM TBS, 1% BSA, 50% Glycerol

Preservative

0.03% Proclin300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

KLH conjugated synthetic phosphopeptide derived from mouse AMPK alpha 2 around the phosphorylation site of Thr172

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX03702

GTX03702 WB Image

WB analysis of mouse cerebral cortex lysates using GTX03702 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Dilution : 1:1000

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Flow cytometry (FACS). GTX03702

GTX03702 FACS Image

FACS analysis of 4% PFA-fixed U-937 cells using GTX03702 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody. Acquisitions of 20,000 events were performed.
Green : Primary antibody
Orange : Isotype control
Permeabilization : 0.1% PBST for 20 min at room temperature
Dilution : 1:100

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX03702

GTX03702 IHC-P Image

IHC-P analysis of rat brain tissue using GTX03702 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15min
Dilution : 1:200

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX03702

GTX03702 WB Image

WB analysis of HepG2 cell lysates using GTX03702 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Dilution : 1:300

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX03702

GTX03702 WB Image

WB analysis of various sample lysates using GTX03702 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Lane 1 : Mouse eye lysates
Lane 2 : Rat muscle lysates
Lane 3 : Rat heart lysates
Lane 4 : Rat kidney lysates
Lane 5 : Rat eye lysates
Dilution : 1:1000

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX03702

GTX03702 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX03702 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15min
Dilution : 1:400

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX03702

GTX03702 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX03702 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15min
Dilution : 1:200

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX03702

GTX03702 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX03702 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15min
Dilution : 1:200

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX03702

GTX03702 IHC-P Image

IHC-P analysis of rat heart tissue using GTX03702 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15min
Dilution : 1:200

REFERENCE

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Package List Price ($)
$ 399