GeneTex
United States (US)

AMPK alpha 2 antibody, Internal

GTX88046_21_ELISA_18122102_651.jpg
GTX88046_2574_WB_18122102_182.jpg
GTX88046_2573_WB_18122102_880.jpg

Cat No. GTX88046

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, sELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
ELISA Assay dependent
sELISA Assay dependent

Note :

Capture : GTX113251 , Detection : GTX88046
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88046-PEP).

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence CPLDALNTTKP, from the internal region of the protein sequence according to NP_006243.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AMPKA2 Antibody , AMPK2 Antibody , PRKAA2 Antibody , AMPK Antibody , PRKAA Antibody

Background

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX88046_21_ELISA_18122102_651.jpg

GTX88046 ELISA Image

ELISA analysis of AMPK alpha 2 protein using GTX88046 AMPK alpha 2 antibody, Internal.
Capture : GTX113251 (2.5μg/ml)
Detection : GTX88046 (1.5μg/ml)

GTX88046_2574_WB_18122102_182.jpg

GTX88046 WB Image

WB analysis of human liver lysate using GTX88046 AMPK alpha 2 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

GTX88046_2573_WB_18122102_880.jpg

GTX88046 WB Image

WB analysis of mouse (A) and rat (B) heart lysate using GTX88046 AMPK alpha 2 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer