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AMPK alpha 2 antibody

Anti-AMPK alpha 2 antibody used in Immunoprecipitation (IP). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487
Anti-AMPK alpha 2 antibody used in Western Blot (WB). GTX103487
Anti-AMPK alpha 2 antibody used in Western Blot (WB). GTX103487
Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

Cat No. GTX103487

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:29753072)

Predict Reactivity

Sheep, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 2 , AMPK , AMPK2 , AMPKa2 , PRKAA

Background

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 2 antibody used in Immunoprecipitation (IP). GTX103487

GTX103487 IP Image

AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 μg of AMPK alpha 2 antibody (GTX103487) 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody (GTX103487) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
AMPK alpha 2 antibody (GTX103487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMPK alpha 2 antibody used in Western Blot (WB). GTX103487

GTX103487 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX103487 diluted at 1:1000

Anti-AMPK alpha 2 antibody used in Western Blot (WB). GTX103487

GTX103487 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 2 antibody (GTX103487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103487

GTX103487 IHC-P Image

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
AMPK alpha 2 antibody (GTX103487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169