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AMPK alpha (phospho Thr172) antibody

Anti-AMPK alpha (phospho Thr172) antibody used in Western Blot (WB). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in Western Blot (WB). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha (phospho Thr172) antibody used in Western Blot (WB). GTX52341

Cat No. GTX52341

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μg ($319)

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200

Calculated MW

64 kDa. ( Note )

Specificity/Sensitivity

AAPK1

PROPERTIES

Form

Liquid

Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172

Purification

Antigen affinity purification

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Protein Kinase Amp-Activated Catalytic Subunit Alpha 1 , Ampk , Ampka1 , Prkaa1

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha (phospho Thr172) antibody used in Western Blot (WB). GTX52341

GTX52341 WB Image

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of human colon tissue using GTX52341 AMPK alpha (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX52341 AMPK alpha (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX52341 AMPK alpha (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of rat skin tissue using GTX52341 AMPK alpha (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX52341 AMPK alpha (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX52341 AMPK alpha (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha (phospho Thr172) antibody used in Western Blot (WB). GTX52341

GTX52341 WB Image

WB analysis of PANC1 cells using GTX52341 AMPK alpha (phospho Thr172) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

Anti-AMPK alpha (phospho Thr172) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52341

GTX52341 ICC/IF Image

ICC/IF analysis of PFA-fixed 293 cells using GTX52341 AMPK alpha (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX52341 AMPK alpha (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX52341 AMPK alpha (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha (phospho Thr172) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52341

GTX52341 ICC/IF Image

ICC/IF analysis of PFA-fixed C2C12 cells using GTX52341 AMPK alpha (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% saponin
Dilution : 1:400

Anti-AMPK alpha (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of rat lung tissue using GTX52341 AMPK alpha (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha (phospho Thr172) antibody used in Western Blot (WB). GTX52341

GTX52341 WB Image

Western blot analysis of AMPK alpha phosphorylation expression in Heatshock treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319