AMPK beta 1 antibody [1A7]

Cat. No. GTX66847

Host	Mouse
Clonality	Monoclonal
Clone Name	1A7
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat, Monkey
Package	100 μl ($459)

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Datasheet
Document

View product citations for antibody GTX66847 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
ICC/IF	1:200
IHC-P	1:800
IP	1:50-1:200

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.2% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human AMPKβ1 protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase AMP-activated non-catalytic subunit beta 1 , AMPK , HAMPKb

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5564 PRKAB1
UniProt: Q9Y478 PRKAB1

Research Area

DATA IMAGES

GTX66847 ICC/IF Image

ICC/IF analysis of 1% PFA-fixed HeLa cells using GTX66847 AMPK beta 1 antibody [1A7].
Dilution : 1:100

GTX66847 WB Image

WB analysis of 3T3, Hela, PC-12, COS7, and MDA-MB-468 cell lysates using GTX66847 AMPK beta 1 antibody [1A7].
Dilution : 1:1000

GTX66847 IHC-P Image

IHC-P analysis of breast cancer tissue using GTX66847 AMPK beta 1 antibody [1A7].
Antigen retrieval : citrate buffer (pH 6.0)

GTX66847 IP Image

IP analysis of HeLa cell lysate using GTX66847 AMPK beta 1 antibody [1A7].

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 459

Qty