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AMPK beta 1 antibody [1A7]

Anti-AMPK beta 1 antibody [1A7] used in Western Blot (WB). GTX66847
Anti-AMPK beta 1 antibody [1A7] used in Immunoprecipitation (IP). GTX66847
Anti-AMPK beta 1 antibody [1A7] used in IHC (Paraffin sections) (IHC-P). GTX66847
Anti-AMPK beta 1 antibody [1A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66847

Cat No. GTX66847

Host

Mouse

Clonality

Monoclonal

Clone Name

1A7

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:200
IHC-P 1:800
IP 1:50-1:200
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine pH 7.4, 150 mM NaCl, 0.2% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human AMPKβ1 protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase AMP-activated non-catalytic subunit beta 1 , AMPK , HAMPKb

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK beta 1 antibody [1A7] used in Western Blot (WB). GTX66847

GTX66847 WB Image

WB analysis of 3T3, Hela, PC-12, COS7, and MDA-MB-468 cell lysates using GTX66847 AMPK beta 1 antibody [1A7].
Dilution : 1:1000

Anti-AMPK beta 1 antibody [1A7] used in Immunoprecipitation (IP). GTX66847

GTX66847 IP Image

IP analysis of HeLa cell lysate using GTX66847 AMPK beta 1 antibody [1A7].

Anti-AMPK beta 1 antibody [1A7] used in IHC (Paraffin sections) (IHC-P). GTX66847

GTX66847 IHC-P Image

IHC-P analysis of breast cancer tissue using GTX66847 AMPK beta 1 antibody [1A7].
Antigen retrieval : citrate buffer (pH 6.0)

Anti-AMPK beta 1 antibody [1A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66847

GTX66847 ICC/IF Image

ICC/IF analysis of 1% PFA-fixed HeLa cells using GTX66847 AMPK beta 1 antibody [1A7].
Dilution : 1:100

REFERENCE

There are currently no references for AMPK beta 1 antibody [1A7] (GTX66847). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for AMPK beta 1 antibody [1A7] (GTX66847). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399