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AMPK gamma 1 antibody

Anti-AMPK gamma 1 antibody used in Western Blot (WB). GTX101661

Cat. No. GTX101661

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all AMPK gamma 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

mouse liver

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AMPK gamma 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036225

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase AMP-activated non-catalytic subunit gamma 1 , AMPKG

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AMPK gamma 1 antibody used in Western Blot (WB). GTX101661

GTX101661 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX101661 diluted at 1:500

REFERENCE

Application Reference

Lin YY et al. Nature 2012; 482 (7384) : 251-5 Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169