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AMPK gamma 1 antibody

Anti-AMPK gamma 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33006
Anti-AMPK gamma 1 antibody used in Western Blot (WB). GTX33006
Anti-AMPK gamma 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33006
Anti-AMPK gamma 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33006
Anti-AMPK gamma 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33006

Cat No. GTX33006

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human PRKAG1 (NP_002724.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Kinase Amp-Activated Non-Catalytic Subunit Gamma 1,Ampkg,Prkag1

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-AMPK gamma 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33006

GTX33006 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX33006 AMPK gamma 1 antibody.
Blue : DAPI

Anti-AMPK gamma 1 antibody used in Western Blot (WB). GTX33006

GTX33006 WB Image

WB analysis of various samples using GTX33006 AMPK gamma 1 antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-AMPK gamma 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33006

GTX33006 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX33006 AMPK gamma 1 antibody.
Dilution : 1:100

Anti-AMPK gamma 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33006

GTX33006 IHC-P Image

IHC-P analysis of rat brain tissue using GTX33006 AMPK gamma 1 antibody.
Dilution : 1:100

Anti-AMPK gamma 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33006

GTX33006 IHC-P Image

IHC-P analysis of human stomach tissue using GTX33006 AMPK gamma 1 antibody.
Dilution : 1:100

REFERENCE
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REVIEW
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Package List Price ($)
$ 329