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AMPK gamma 2 antibody [C2C3], C-term

Anti-AMPK gamma 2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114178
Anti-AMPK gamma 2 antibody [C2C3], C-term used in Western Blot (WB). GTX114178
Anti-AMPK gamma 2 antibody [C2C3], C-term used in Western Blot (WB). GTX114178

Cat. No. GTX114178

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all AMPK gamma 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

Mouse brain , HepG2

Predict Reactivity

Rat, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human AMPK gamma 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623612

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase AMP-activated non-catalytic subunit gamma 2 , AAKG , AAKG2 , CMH6 , H91620p , WPWS

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AMPK gamma 2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114178

GTX114178 IHC-P Image

AMPK gamma 2 antibody [C2C3], C-term detects PRKAG2 protein at nucleus and cytosol human on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
AMPK gamma 2 antibody [C2C3], C-term (GTX114178) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AMPK gamma 2 antibody [C2C3], C-term used in Western Blot (WB). GTX114178

GTX114178 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX114178 diluted at 1:1000

Anti-AMPK gamma 2 antibody [C2C3], C-term used in Western Blot (WB). GTX114178

GTX114178 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK gamma 2 antibody [C2C3], C-term (GTX114178) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lin YY et al. Nature 2012; 482 (7384) : 251-5 Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169