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AMPK1/AMPK2 (phospho Ser485/Ser491) antibody

Anti-AMPK1/AMPK2 (phospho Ser485/Ser491) antibody used in Western Blot (WB). GTX79142
Anti-AMPK1/AMPK2 (phospho Ser485/Ser491) antibody used in IHC (Paraffin sections) (IHC-P). GTX79142

Cat. No. GTX79142

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of serine 485/491 (S-G- Sp-V-S).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11173037

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AMPK,AMPKa1,PRKAA1,protein kinase AMPactivated catalytic subunit alpha 1,AMPK alpha 1,protein kinase AMP-activated catalytic subunit alpha 1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK1/AMPK2 (phospho Ser485/Ser491) antibody used in Western Blot (WB). GTX79142

GTX79142 WB Image

WB analysis of HeLa cells untreated or treated with heat shock (30min) lysates using GTX79142 AMPK1/AMPK2 (phospho Ser485/Ser491) antibody.

Anti-AMPK1/AMPK2 (phospho Ser485/Ser491) antibody used in IHC (Paraffin sections) (IHC-P). GTX79142

GTX79142 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX79142 AMPK1/AMPK2 (phospho Ser485/Ser491) antibody.

REFERENCE

There are currently no references for AMPK1/AMPK2 (phospho Ser485/Ser491) antibody (GTX79142). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 399