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ANGPTL3 antibody

Orthogonal
Anti-ANGPTL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104569
Anti-ANGPTL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104569
Anti-ANGPTL3 antibody used in Western Blot (WB). GTX104569

Cat. No. GTX104569

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all ANGPTL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ANGPTL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167236

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiopoietin like 3 , ANG-5 , ANGPT5 , ANL3 , FHBL2

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ANGPTL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104569

GTX104569 IHC-P Image

ANGPTL3 antibody detects ANGPTL3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
ANGPTL3 stained by ANGPTL3 antibody (GTX104569) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ANGPTL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104569

GTX104569 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ANGPTL3(GTX104569) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ANGPTL3 antibody used in Western Blot (WB). GTX104569

GTX104569 WB Image

Orthogonal

HepG2 conditioned medium (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ANGPTL3 antibody (GTX104569) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

I Rossi et al. Int J Mol Sci 2024; 25 (7) : 3708 Gene Silencing of Angiopoietin-like 3 (ANGPTL3) Induced De Novo Lipogenesis and Lipid Accumulation in Huh7 Cell Line
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169
Custom Conjugations