GeneTex
United States (US)

ANKHD1 antibody [N1], N-term

GTX115568_40191_WB_2_18121410_599.jpg
GTX115568_WB_18121410_619.jpg
GTX115568_40191_IHC_18121410_574.jpg

Cat No. GTX115568

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

269 kDa. ( Note )

Positive Control

A549 , HepG2 , HCT116 , Jurkat , Raji , NCI-H929

Predict Reactivity

Mouse, Rat, Dog, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ANKHD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ankyrin Repeat And Kh Domain Containing 1,Mask,Mask1,Pp2500,Vbarp,Ankhd1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. Co-transcription of this gene and the neighboring downstream gene (EIF4EBP3) generates a transcript (MASK-BP3) which encodes a fusion protein comprised of the MASK protein sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115568_40191_WB_2_18121410_599.jpg

GTX115568 WB Image

ANKHD1 antibody [N1], N-term detects ANKHD1 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
ANKHD1 antibody [N1], N-term (GTX115568) dilution: 1:1000

GTX115568_WB_18121410_619.jpg

GTX115568 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
ANKHD1 antibody
GTX115568 diluted at 1:1000

GTX115568_40191_IHC_18121410_574.jpg

GTX115568 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ANKHD1(GTX115568) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169