APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Note :
Sandwich ELISA
Capture: GTX634219, Detection: GTX639071
|
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human ANP recombinant protein.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888430
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
natriuretic peptide A , ANF , ANP , ATFB6 , ATRST2 , CDD , CDD-ANF , CDP , PND
Cellular Localization
Secreted
Background
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX634219 IHC-P Image
ANP antibody [GT1068] detects secreted ANP protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.ANP stained by ANP antibody [GT1068] (GTX634219) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX634219 WB Image
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [GT1068] (GTX634219) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX634219 ICC/IF Image
ANP antibody [GT1068] detects ANP protein at cytoplasm by immunofluorescent analysis.Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ANP stained by ANP antibody [GT1068] (GTX634219) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
|
|
GTX634219 ELISA Image
Sandwich ELISA detection of recombinant Human ANP protein, GST tag (GTX109255-pro) using antibodies as below. Capture: ANP antibody [GT1068] (GTX634219) (5 μg/mL) Detection: ANP antibody [HL2617] (GTX639071) (1 μg/mL)
|
REFERENCE
There are currently no references for ANP antibody [GT1068] (GTX634219). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ANP antibody [GT1068] (GTX634219). Be the first to share your experience with this product.