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ANP antibody [HL2617]

Recombinant antibody
Anti-ANP antibody [HL2617] used in Western Blot (WB). GTX639071
Anti-ANP antibody [HL2617] used in Western Blot (WB). GTX639071
Anti-ANP antibody [HL2617] used in Western Blot (WB). GTX639071
Anti-ANP antibody [HL2617] used in ELISA (ELISA). GTX639071
Anti-ANP antibody [HL2617] used in IHC (Paraffin sections) (IHC-P). GTX639071

Cat. No. GTX639071

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2617

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ANP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX634219, Detection: GTX639071
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ANP recombinant protein

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

natriuretic peptide A , ANF , ANP , ATFB6 , ATRST2 , CDD , CDD-ANF , CDP , PND

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-ANP antibody [HL2617] used in Western Blot (WB). GTX639071

GTX639071 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [HL2617] (GTX639071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ANP antibody [HL2617] used in Western Blot (WB). GTX639071

GTX639071 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [HL2617] (GTX639071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ANP antibody [HL2617] used in Western Blot (WB). GTX639071

GTX639071 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [HL2617] (GTX639071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ANP antibody [HL2617] used in ELISA (ELISA). GTX639071

GTX639071 ELISA Image

Sandwich ELISA detection of recombinant Human ANP protein, GST tag (GTX109255-pro) using antibodies as below.

Capture: ANP antibody [GT1068] (GTX634219) (5 μg/mL)
Detection: ANP antibody [HL2617] (GTX639071) (1 μg/mL)

Anti-ANP antibody [HL2617] used in IHC (Paraffin sections) (IHC-P). GTX639071

GTX639071 IHC-P Image

ANP antibody [HL2617] detects ANP protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
ANP stained by ANP antibody [HL2617] (GTX639071) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations