APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:20000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human ANP Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11168116
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
natriuretic peptide A , ANF , ANP , ATFB6 , ATRST2 , CDD , CDD-ANF , CDP , PND
Cellular Localization
Secreted
Background
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX112698 IHC-P Image
ANP antibody [N1C3] detects secreted ANP protein by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.ANP stained by ANP antibody [N1C3] (GTX112698) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112698 IHC-P Image
ANP antibody [N1C3] detects ANP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.ANP stained by ANP antibody [N1C3] (GTX112698) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112698 IHC-P Image
ANP antibody [N1C3] detects ANP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.ANP stained by ANP antibody [N1C3] (GTX112698) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112698 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [N1C3] (GTX112698) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112698 IHC-P Image
ANP antibody [N1C3] detects ANP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.ANP stained by ANP antibody [N1C3] (GTX112698) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112698 IHC-P Image
ANP antibody [N1C3] detects ANP protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ANP antibody [N1C3] (GTX112698) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112698 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ANP(GTX112698) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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REVIEW
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