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ANP antibody

Orthogonal
Anti-ANP antibody used in IHC (Paraffin sections) (IHC-P). GTX109255
Anti-ANP antibody used in IHC (Paraffin sections) (IHC-P). GTX109255
Anti-ANP antibody used in Western Blot (WB). GTX109255
Anti-ANP antibody used in IHC (Paraffin sections) (IHC-P). GTX109255
Anti-ANP antibody used in Western Blot (WB). GTX109255
Anti-ANP antibody used in Western Blot (WB). GTX109255

Cat. No. GTX109255

Reference 12

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Frog, Squirrel
Package
100 μl ($399),
25 μl ($169)
See all ANP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

mouse heart

Predict Reactivity

Rabbit, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ANP Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729584

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

natriuretic peptide A , ANF , ANP , ATFB6 , ATRST2 , CDD , CDD-ANF , CDP , PND

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ANP antibody used in IHC (Paraffin sections) (IHC-P). GTX109255

GTX109255 IHC-P Image

ANP antibody detects ANP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ANP stained by ANP antibody (GTX109255) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ANP antibody used in IHC (Paraffin sections) (IHC-P). GTX109255

GTX109255 IHC-P Image

ANP antibody detects ANP protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
ANP antibody (GTX109255) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ANP antibody used in Western Blot (WB). GTX109255

GTX109255 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody (GTX109255) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ANP antibody used in IHC (Paraffin sections) (IHC-P). GTX109255

GTX109255 IHC-P Image

ANP antibody detects ANP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
ANP stained by ANP antibody (GTX109255) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ANP antibody used in Western Blot (WB). GTX109255

GTX109255 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2019. PMID: 30816171

Anti-ANP antibody used in Western Blot (WB). GTX109255

GTX109255 WB Image

The data was published in the journal PLoS One in 2015.PMID: 25679215

REFERENCE

Application Reference

Nandi SS et al. Front Physiol 2023; 14 : 1277065 Enhanced central sympathetic tone induces heart failure with preserved ejection fraction (HFpEF) in rats.
Gu X et al. Biology (Basel) 2023; 12 (7) Corin Deficiency Diminishes Intestinal Sodium Excretion in Mice.
Gu, X et al. Biology 2023; 12 : 945 Corin Deficiency Diminishes Intestinal Sodium Excretion in Mice
Application : IHC-P
Reactivity : Mouse
Zhao T et al. Oxid Med Cell Longev 2022; 2022 : 6227330 Hdac8 Inhibitor Alleviates Transverse Aortic Constriction-Induced Heart Failure in Mice by Downregulating Ace1.
Application : WB
Nandi SS et al. Front Physiol 2021; 12 : 718982 Neurogenic Hypertension Mediated Mitochondrial Abnormality Leads to Cardiomyopathy: Contribution of UPRmt and Norepinephrine-miR- 18a-5p-HIF-1α Axis.
Application : ICC/IF
Reactivity : Rat
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REVIEW

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Application Reference
Nandi SS et al. Front Physiol 2023; 14:1277065 Enhanced central sympathetic tone induces heart failure with preserved ejection fraction (HFpEF) in rats.
Gu X et al. Biology (Basel) 2023; 12 (7) Corin Deficiency Diminishes Intestinal Sodium Excretion in Mice.
Gu, X et al. Biology 2023; 12:945 Corin Deficiency Diminishes Intestinal Sodium Excretion in Mice
Application : IHC-P
Reactivity : Mouse
Zhao T et al. Oxid Med Cell Longev 2022; 2022:6227330 Hdac8 Inhibitor Alleviates Transverse Aortic Constriction-Induced Heart Failure in Mice by Downregulating Ace1.
Application : WB
Nandi SS et al. Front Physiol 2021; 12:718982 Neurogenic Hypertension Mediated Mitochondrial Abnormality Leads to Cardiomyopathy: Contribution of UPRmt and Norepinephrine-miR- 18a-5p-HIF-1α Axis.
Application : ICC/IF
Reactivity : Rat
Zhao T et al. Front Pharmacol 2021; 12:677757 Selective HDAC8 Inhibition Attenuates Isoproterenol-Induced Cardiac Hypertrophy and Fibrosis via p38 MAPK Pathway.
Reactivity : Mouse
Wen J et al. Int J Mol Med 2020; 45 (3):873-885 Pyrroloquinoline quinone attenuates isoproterenol hydrochloride?induced cardiac hypertrophy in AC16 cells by inhibiting the NF?κB signaling pathway.
Sun S et al. Sci Rep 2019; 9 (1):3018 Gentisic acid prevents the transition from pressure overload-induced cardiac hypertrophy to heart failure.
Jia D et al. J Cell Physiol 2019; (Epub) Postinfarction exercise training alleviates cardiac dysfunction and adverse remodeling via mitochondrial biogenesis and SIRT1/PGC-1帢/PI3K/Akt signaling.
Application : WB
Reactivity : Rat
Al-Attar R et al. Cell Biochem Funct 2018; 36 (8):420-430 Effects of anoxic exposure on the nuclear factor of activated T cell (NFAT) transcription factors in the stress-tolerant wood frog.
Application : WB
Reactivity : Frog
Kesherwani V et al. Mol Cell Biochem 2015; 404 (43102):241-50 Hydrogen sulfide mitigates homocysteine-mediated pathological remodeling by inducing miR-133a in cardiomyocytes.
Application : WB
Reactivity : Human
Luu BE et al. PLoS One 2015; 10 (2):e0117747 The regulation of troponins I, C and ANP by GATA4 and Nkx2-5 in heart of hibernating thirteen-lined ground squirrels, Ictidomys tridecemlineatus.
Application : WB
Reactivity : Squirrel
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169