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ANP32A antibody [N1C2]

Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933
Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933
Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933
Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933
Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933

Cat No. GTX115933

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , mouse brain

Product Note

Based on sequence homology, this antibody may cross react with ANP32B or ANP32C proteins.

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ANP32A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624431

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acidic nuclear phosphoprotein 32 family member A , C15orf1 , HPPCn , I1PP2A , LANP , MAPM , PHAP1 , PHAPI , PP32

Cellular Localization

Nucleus , Cytoplasm

Background

Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.

Database

Research Area

DATA IMAGES

Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933

GTX115933 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ANP32A(GTX115933) antibody at 1:200 dilution.

Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933

GTX115933 IHC-P Image

ANP32A antibody [N1C2] detects ANP32A protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ANP32A antibody [N1C2] (GTX115933) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933

GTX115933 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX115933 diluted at 1:1000

Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933

GTX115933 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX115933 diluted at 1:1000

Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933

GTX115933 IHC-P Image

ANP32A antibody [N1C2] detects ANP32A protein at nucleus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ANP32A antibody [N1C2] (GTX115933) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for ANP32A antibody [N1C2] (GTX115933). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ANP32A antibody [N1C2] (GTX115933). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169