APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , Raji
Product Note
Based on sequence homology, this antibody may cross react with ANP32B or ANP32C proteins.
Predict Reactivity
Rat, Zebrafish, Bovine, Dog, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ANP32A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10624431
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
acidic nuclear phosphoprotein 32 family member A , C15orf1 , HPPCn , I1PP2A , LANP , MAPM , PHAP1 , PHAPI , PP32
Cellular Localization
Nucleus , Cytoplasm
Background
Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
Database
Research Area
DATA IMAGES
![Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933](/templates/default/js/lightbox/images/loading.gif "Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933")
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GTX115933 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ANP32A(GTX115933) antibody at 1:200 dilution.
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GTX115933 IHC-P Image
ANP32A antibody [N1C2] detects ANP32A protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ANP32A antibody [N1C2] (GTX115933) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX115933 WB Image
Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX115933 diluted at 1:1000
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GTX115933 WB Image
Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX115933 diluted at 1:1000
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GTX115933 IHC-P Image
ANP32A antibody [N1C2] detects ANP32A protein at nucleus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ANP32A antibody [N1C2] (GTX115933) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for ANP32A antibody [N1C2] (GTX115933). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ANP32A antibody [N1C2] (GTX115933). Be the first to share your experience with this product.
![Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933](/upload/website/prouct_img/small/GTX115933/GTX115933_40289_IFA_w_23060519_522.webp "Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933")
![Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933](/upload/website/prouct_img/small/GTX115933/GTX115933_40289_20150714_IHC-P_M_w_23060519_365.webp "Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933")
![Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933](/upload/website/prouct_img/small/GTX115933/GTX115933_WB_w_23060519_440.webp "Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933")
![Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933](/upload/website/prouct_img/small/GTX115933/GTX115933_40289_WB_M_brain_w_23060519_590.webp "Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933")
![Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933](/upload/website/prouct_img/small/GTX115933/GTX115933_40289_20150714_IHC-P_M_2_w_23060519_147.webp "Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933")
![Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933](/upload/website/prouct_img/normal/GTX115933/GTX115933_40289_IFA_w_23060519_522.webp "Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933")
![Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933](/upload/website/prouct_img/normal/GTX115933/GTX115933_40289_20150714_IHC-P_M_w_23060519_365.webp "Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933")
![Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933](/upload/website/prouct_img/normal/GTX115933/GTX115933_WB_w_23060519_440.webp "Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933")
![Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933](/upload/website/prouct_img/normal/GTX115933/GTX115933_40289_WB_M_brain_w_23060519_590.webp "Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933")
![Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933](/upload/website/prouct_img/normal/GTX115933/GTX115933_40289_20150714_IHC-P_M_2_w_23060519_147.webp "Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933")