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ANP32B antibody

Anti-ANP32B antibody used in IHC (Paraffin sections) (IHC-P). GTX115437
Anti-ANP32B antibody used in Western Blot (WB). GTX115437

Cat. No. GTX115437

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ANP32B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ANP32B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164867

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acidic nuclear phosphoprotein 32 family member B , APRIL , PHAPI2 , SSP29

Cellular Localization

Nucleus

Background

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity.

Database

Research Area

DATA IMAGES

Anti-ANP32B antibody used in IHC (Paraffin sections) (IHC-P). GTX115437

GTX115437 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ANP32B(GTX115437) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ANP32B antibody used in Western Blot (WB). GTX115437

GTX115437 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
12% SDS PAGE
GTX115437 diluted at 1:1000

REFERENCE

Application Reference

Yang LL et al. Heliyon 2024; 10 (1) : e23829 ANP32B promotes colorectal cancer cell progression and reduces cell sensitivity to PRAP1 inhibitor through up-regulating HPF1.
LL Yang et al. Heliyon 2024; 10 (1) : e23829 ANP32B promotes colorectal cancer cell progression and reduces cell sensitivity to PRAP1 inhibitor through up-regulating HPF1
Application : WB
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169