GeneTex
United States (US)

ANP32B antibody

GTX115437_40520_IHC_18121410_120.jpg
GTX115437_40520_WB_18121410_768.jpg

Cat No. GTX115437

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , Jurkat

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ANP32B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acidic Nuclear Phosphoprotein 32 Family Member B,April,Phapi2,Ssp29,Anp32B

Cellular Localization

Nucleus

Background

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity.

Database

Research Area

DATA IMAGES
GTX115437_40520_IHC_18121410_120.jpg

GTX115437 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ANP32B(GTX115437) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115437_40520_WB_18121410_768.jpg

GTX115437 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
12% SDS PAGE
GTX115437 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169