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AP chromogen BCIP/NBT (ready-to-use)

Cat. No. GTX27468

Reference 1

Application

 WB, IHC-P, IHC-Fr, Dot
Package
100 ml ($209)
See all Chromogen : BCIP/NBT products
PRODUCT

Summary

Alkaline Phosphatase chromogen BCIP/NBT (ready-to-use)

APPLICATION

Application Note

See ProtocolBCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light.

PROPERTIES

Form

Liquid

Storage

Discard if the solution turns purple or turbid. The reagent is stable at room temperature, but we recommend storage in 2ºC to 8ºC to increase the shelf life. Warm to assay temperature before use.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCIP NBT

Background

Alkaline Phosphatase based immunostaining systems are gaining wide acceptance among pathologists because of their relatively high signal to noise ratio. 5-bromo,4-chloro,3-indolylphosphate (BCIP)/nitroblue tetrazolium (NBT) substrate for alkaline phosphatase-based detection systems, is a commonly used substrate chromogen. BCIP acts as the substrate for alkaline phosphatase, and the NBT enhances the purplish-brown final color of the precipitates at the reaction sites.

REFERENCE

Application Reference

Moser K et al. Arthritis Rheum 2012; 64 (4) : 1237-46 CXCR3 promotes the production of IgG1 autoantibodies but is not essential for the development of lupus nephritis in NZB/NZW mice.
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REVIEW

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Package List Price ($)
$ 209