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AP1G1 antibody

Anti-AP1G1 antibody used in Western Blot (WB). GTX23706
Anti-AP1G1 antibody used in Western Blot (WB). GTX23706

Cat. No. GTX23706

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Human
Package
100 μg ($589)
See all gamma Adaptin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IP 1:100
ELISA 1:2000-1:8000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region near aa 625-675 of Human AP1G1.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adaptor related protein complex 1 subunit gamma 1 , ADTG , CLAPG1

Cellular Localization

Golgi apparatus

Background

AP1G1 is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Database

Research Area

DATA IMAGES

Anti-AP1G1 antibody used in Western Blot (WB). GTX23706

GTX23706 WB Image

Western blot using GeneTex Affinity Purified anti-AP1G1 antibody (GTX23706) shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 μg/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 μg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.

Anti-AP1G1 antibody used in Western Blot (WB). GTX23706

GTX23706 WB Image

WB analysis of HeLa whole cell lysate using GTX23706 AP1G1 antibody prior incubated with (Lane 2) or without (Lane 1) blocking peptide.
Loading : 20 μg
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589