WB analysis of HeLa whole cell lysate using GTX23706 AP1G1 antibody prior incubated with (Lane 2) or without (Lane 1) blocking peptide.
Loading : 20 μg
Dilution : 1:500

Western blot using GeneTex Affinity Purified anti-AP1G1 antibody (GTX23706) shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 μg/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 μg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.