GeneTex
United States (US)

AP2 alpha antibody [3B5]

GTX15878_1050_IHC-P_19011519_427.jpg
GTX15878_344_ICC-IF_19011519_441.jpg
GTX15878_345_ICC-IF_19011519_356.jpg
GTX15878_343_ICC-IF_19011519_852.jpg
GTX15878_1549_WB_19011519_506.jpg

Cat No. GTX15878

Host Mouse
Clonality Monoclonal
Clone Name 3B5
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IP, IHC
Reactivity Human, Mouse, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:50 - 1:200
ICC/IF 1:50-1:500
IHC-P 1:50
IP 1:100 - 1:250
IHC Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

N-terminus of human AP2a.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transcription Factor Ap-2 Alpha,Ap-2,Ap-2Alpha,Ap2Tf,Bofs,Tfap2,Tfap2A

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES
GTX15878_1050_IHC-P_19011519_427.jpg

GTX15878 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX15878 AP2 alpha antibody [3B5].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

GTX15878_344_ICC-IF_19011519_441.jpg

GTX15878 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 °C

GTX15878_345_ICC-IF_19011519_356.jpg

GTX15878 ICC/IF Image

ICC/IF analysis of U251 cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 °C

GTX15878_343_ICC-IF_19011519_852.jpg

GTX15878 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4°C

GTX15878_1549_WB_19011519_506.jpg

GTX15878 WB Image

WB analysis of whole cell extracts (30 µg lysate) of PC-3 (Lane1), A-431 (Lane2), Mouse Placenta (Lane3) and RAW 264.7 (Lane5) using GTX15878 AP2 alpha antibody [3B5].
Dilution : 1:50-1:200

Package List Price ($)
$ 319