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AP2 beta antibody

Anti-AP2 beta antibody used in Immunoprecipitation (IP). GTX114832
Anti-AP2 beta antibody used in Western Blot (WB). GTX114832
Anti-AP2 beta antibody used in Western Blot (WB). GTX114832

Cat. No. GTX114832

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all AP2 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

MCF-7 , rat brain

Predict Reactivity

Mouse, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AP2 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163339

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor AP-2 beta , AP-2B , AP2-B , PDA2

Cellular Localization

Nucleus

Background

This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AP2 beta antibody used in Immunoprecipitation (IP). GTX114832

GTX114832 IP Image

AP2 beta antibody immunoprecipitates AP2 beta protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. 30 μg MCF-7 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of AP2 beta protein by 4 μg AP2 beta antibody (GTX114832)
7.5 % SDS-PAGE
The immunoprecipitated AP2 beta protein was detected by AP2 beta antibody (GTX114832) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-AP2 beta antibody used in Western Blot (WB). GTX114832

GTX114832 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AP2 beta antibody (GTX114832) diluted at 1:500.

Anti-AP2 beta antibody used in Western Blot (WB). GTX114832

GTX114832 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX114832 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169