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AP2 gamma antibody

Orthogonal Overexpression
Anti-AP2 gamma antibody used in Western Blot (WB). GTX134259
Anti-AP2 gamma antibody used in Western Blot (WB). GTX134259

Cat. No. GTX134259

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AP2 gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AP2 gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.
Glycerol|PBS|ProClin

Conjugation

Unconjugated

RRID

AB_2887250

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor AP-2 gamma , AP2-GAMMA , ERF1 , TFAP2G , hAP-2g

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a sequence-specific DNA-binding transcription factor involved in the activation of several developmental genes. The encoded protein can act as either a homodimer or heterodimer with other family members and is induced during retinoic acid-mediated differentiation. It plays a role in the development of the eyes, face, body wall, limbs, and neural tube. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AP2 gamma antibody used in Western Blot (WB). GTX134259

GTX134259 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AP2 gamma antibody (GTX134259) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AP2 gamma antibody used in Western Blot (WB). GTX134259

GTX134259 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AP2 gamma antibody (GTX134259) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 22964634

REFERENCE

Application Reference

Damian Ko?at et al. Cancers (Basel) 2021; 13 (12) WWOX Loses the Ability to Regulate Oncogenic AP-2γ and Synergizes with Tumor Suppressor AP-2α in High-Grade Bladder Cancer.
Application : WB
Kołat D. et al. Cancers 2021; 13 (12) : 2957 WWOX Loses the Ability to Regulate Oncogenic AP-2γ and Synergizes with Tumor Suppressor AP-2α in High-Grade Bladder Cancer.
Application : WB
Reactivity : Human
Kołat D. et al. Front Oncol. 2021; 11 : 621060 Fragile Gene WWOX Guides TFAP2A/ TFAP2C-Dependent Actions Against Tumor Progression in Grade II Bladder Cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169