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AP2A1 antibody

Anti-AP2A1 antibody used in Western Blot (WB). GTX32449
Anti-AP2A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32449
Anti-AP2A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32449

Cat No. GTX32449

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human AP2A1 (NP_055018.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

adaptor related protein complex 2 subunit alpha 1 , ADTAA , AP2-ALPHA , CLAPA1

Cellular Localization

Cell membrane

Background

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AP2A1 antibody used in Western Blot (WB). GTX32449

GTX32449 WB Image

WB analysis of various samples using GTX32449 AP2A1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-AP2A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32449

GTX32449 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX32449 AP2A1 antibody.
Dilution : 1:100

Anti-AP2A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32449

GTX32449 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX32449 AP2A1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 329