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AP2B1 antibody

Anti-AP2B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79316
Anti-AP2B1 antibody used in Western Blot (WB). GTX79316
Anti-AP2B1 antibody used in Western Blot (WB). GTX79316

Cat. No. GTX79316

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μg ($399)
See all AP2B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/mL
ICC/IF 2 μg/ml
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues I(588) H R K H L P I H H G S T D A G D S P(606) of human beta Adaptin 2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11165166

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adaptor related protein complex 2 subunit beta 1 , ADTB2 , AP105B , AP2-BETA , CLAPB1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AP2B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79316

GTX79316 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX79316 AP2B1 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-AP2B1 antibody used in Western Blot (WB). GTX79316

GTX79316 WB Image

WB analysis of bovine brain using GTX79316 AP2B1 antibody.

Anti-AP2B1 antibody used in Western Blot (WB). GTX79316

GTX79316 WB Image

WB analysis of membrane enriched and tissue extract (30 ug) of SH-SY5Y (Lane 1), PC-3 (Lane 2) and Mouse Brain (Lane 3) using GTX79316 AP2B1 antibody.
Dilution : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399