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AP2M1 antibody

KO/KD Orthogonal
Anti-AP2M1 antibody used in Western Blot (WB). GTX113332
Anti-AP2M1 antibody used in Western Blot (WB). GTX113332
Anti-AP2M1 antibody used in Western Blot (WB). GTX113332

Cat. No. GTX113332

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all AP2M1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HepG2 , Mouse brain

Predict Reactivity

Rat, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AP2M1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949668

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adaptor related protein complex 2 subunit mu 1 , AP50 , CLAPM1 , mu2

Cellular Localization

Cell membrane , Membrane , coated pit

Background

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AP2M1 antibody used in Western Blot (WB). GTX113332

GTX113332 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AP2M1 antibody (GTX113332) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-AP2M1 antibody used in Western Blot (WB). GTX113332

GTX113332 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113332 diluted at 1:500

Anti-AP2M1 antibody used in Western Blot (WB). GTX113332

GTX113332 WB Image

KO/KD

The data was published in the journal Elife in 2019.PMID: 30801249

REFERENCE

Application Reference

HN Huang et al. Int J Mol Sci 2024; 25 (3) : 1374 Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer
Application : WB
Rivera-Serrano EE et al. Elife 2019; 8 Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses.
Bellomo F et al. PLoS One 2016; 11 (5) : e0154805 Carboxyl-Terminal SSLKG Motif of the Human Cystinosin-LKG Plays an Important Role in Plasma Membrane Sorting.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169