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AP2M1 antibody

Anti-AP2M1 antibody used in Western Blot (WB). GTX85110
Anti-AP2M1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85110
Anti-AP2M1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85110

Cat. No. GTX85110

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all AP2M1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Human Kidney Tissue Lysate(GTX29995)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

AP2M1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human AP2M1.The immunogen is located within amino acids 360 - 410 of AP2M1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adaptor related protein complex 2 subunit mu 1 , AP50 , CLAPM1 , mu2

Cellular Localization

Cell membrane

Background

The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.

Database

Research Area

DATA IMAGES

Anti-AP2M1 antibody used in Western Blot (WB). GTX85110

GTX85110 WB Image

WB analysis of human kidney tissue lysate using GTX85110 AP2M1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-AP2M1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85110

GTX85110 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX85110 AP2M1 antibody.
Working concentration : 2.5 μg/ml

Anti-AP2M1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85110

GTX85110 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX85110 AP2M1 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399