GeneTex
United States (US)

AP4M1 antibody [N1C1]

GTX115196_40254_20150715_IHC-P_M_18121410_976.jpg
GTX115196_WB_18121410_722.jpg
GTX115196_40254_IHC_18121410_479.jpg

Cat No. GTX115196

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AP4M1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adaptor Related Protein Complex 4 Subunit Mu 1,Cpsq3,Mu-4,Mu-Arp2,Spg50,Ap4M1

Cellular Localization

Golgi apparatus , trans-Golgi network , Membrane , coated pit

Background

This gene encodes a subunit of the heterotetrameric AP-4 complex. The encoded protein belongs to the adaptor complexes medium subunits family. This AP-4 complex is involved in the recognition and sorting of cargo proteins with tyrosine-based motifs from the trans-golgi network to the endosomal-lysosomal system. [provided by RefSeq]

Database

DATA IMAGES
GTX115196_40254_20150715_IHC-P_M_18121410_976.jpg

GTX115196 IHC-P Image

AP4M1 antibody [N1C1] detects AP4M1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
AP4M1 antibody [N1C1] (GTX115196) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115196_WB_18121410_722.jpg

GTX115196 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX115196 diluted at 1:1000

GTX115196_40254_IHC_18121410_479.jpg

GTX115196 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using AP4M1(GTX115196) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169