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APAF1 antibody [C3], C-term

Anti-APAF1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101067
Anti-APAF1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101067
Anti-APAF1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101067

Cat No. GTX101067

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all APAF1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

Target recombinant protein

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human APAF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

apoptotic peptidase activating factor 1,APAF-1,CED4

Cellular Localization

Cytoplasm

Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-APAF1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101067

GTX101067 IHC-P Image

APAF1 antibody [C3], C-term detects APAF1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
APAF1 antibody [C3], C-term (GTX101067) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-APAF1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101067

GTX101067 IHC-P Image

APAF1 antibody [C3], C-term detects APAF1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
APAF1 antibody [C3], C-term (GTX101067) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-APAF1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101067

GTX101067 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using APAF1(GTX101067) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169