GeneTex
United States (US)

APAF1 antibody [C3], C-term

GTX101067_40359_20160713_IHC-P_M_19011506_838.jpg
GTX101067_40359_20160713_IHC-P_R_19011506_288.jpg
GTX101067_40359_IHC_19011506_685.jpg

Cat No. GTX101067

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

Target recombinant protein

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human APAF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APAF-1 antibody, CED4 antibody, DKFZp781B1145 antibody, APAF1 antibody, apoptotic protease-activating factor 1 antibody, apoptotic peptidase activating factor 1 antibody

Cellular Localization

Cytoplasm

Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101067_40359_20160713_IHC-P_M_19011506_838.jpg

GTX101067 IHC-P Image

APAF1 antibody [C3], C-term detects APAF1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
APAF1 antibody [C3], C-term (GTX101067) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101067_40359_20160713_IHC-P_R_19011506_288.jpg

GTX101067 IHC-P Image

APAF1 antibody [C3], C-term detects APAF1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
APAF1 antibody [C3], C-term (GTX101067) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101067_40359_IHC_19011506_685.jpg

GTX101067 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using APAF1(GTX101067) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf