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APAF1 antibody

Anti-APAF1 antibody used in Flow cytometry (FACS). GTX04330
Anti-APAF1 antibody used in Western Blot (WB). GTX04330

Cat. No. GTX04330

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($469)
See all APAF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/ml
FACS 1-3 μg/1x10⁶ cells
ELISA 0.1-0.5 μg/ml
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human APAF1 recombinant protein (Position: M1-E1248)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apoptotic peptidase activating factor 1 , APAF-1 , CED4

Cellular Localization

Cytoplasm

Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-APAF1 antibody used in Flow cytometry (FACS). GTX04330

GTX04330 FACS Image

FACS analysis of U937 cells using GTX04330 APAF1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled cell
Antibody amount : 1μg/1x10⁶ cells

Anti-APAF1 antibody used in Western Blot (WB). GTX04330

GTX04330 WB Image

WB analysis of various cell lysates using GTX04330 APAF1 antibody.
Lane 1: HEL whole cell lysates
Lane 2: HL-60 whole cell lysates
Dilution : 0.5μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 469