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APAF1 antibody

Anti-APAF1 antibody used in Western Blot (WB). GTX22000
Anti-APAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX22000

Cat. No. GTX22000

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($479)
See all APAF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-10μg/ml
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to aa 1158- 1177 of human Apaf1. This sequence differs from that of mouse Apaf1 by one amino acid.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_384717

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

APAF1 , CED4 , apoptotic peptidase activating factor 1

Cellular Localization

Cytoplasm

Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-APAF1 antibody used in Western Blot (WB). GTX22000

GTX22000 WB Image

Western blot analysis of Apaf1 in human heart tissue lysate with Apaf1 (CT) antibody (GTX22000) at 1μg/ml in the absence (A) or presence (B) of blocking peptide.

Anti-APAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX22000

GTX22000 IHC-P Image

Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 (CT) antibody (GTX22000) at 20 μg/ml.

REFERENCE

Application Reference

Lu CC et al. Int J Oncol 2019; 54 (4) : 1271-1281
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 479