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APAF1 antibody

Anti-APAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22001
Anti-APAF1 antibody used in Western Blot (WB). GTX22001
Anti-APAF1 antibody used in Immunohistochemistry (IHC). GTX22001
Anti-APAF1 antibody used in Western Blot (WB). GTX22001

Cat. No. GTX22001

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($479)
See all APAF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
IHC 1-2μg/ml
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to aa 12-28 of human Apaf1. This sequence is identical in human and mouse.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

APAF1 , CED4 , apoptotic peptidase activating factor 1

Cellular Localization

Cytoplasm

Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-APAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22001

GTX22001 ICC/IF Image

ICC/IF analysis of K562 cells using GTX22001 APAF1 antibody.
Dilution : 10 μg/ml

Anti-APAF1 antibody used in Western Blot (WB). GTX22001

GTX22001 WB Image

WB analysis of K562 cell lysate using GTX22001 APAF1 antibody.
Dilution : 1μg/ml (Lane A) and 2 μg/ml (Lane B)

Anti-APAF1 antibody used in Immunohistochemistry (IHC). GTX22001

GTX22001 IHC Image

Immunohistochemical staining of human heart with Rabbit anti APAF1 (GTX22001)

Anti-APAF1 antibody used in Western Blot (WB). GTX22001

GTX22001 WB Image

Western Blot analysis of whole cell lysate from K562 cells probed with Rabbit anti APAF1 (GTX22001) either in the absence (A) or presence (B) of blocking peptide

REFERENCE

Application Reference

Zhen Tian et al. Cell Cycle 2021; 20 (9) : 874-893 Promoting roles of KLF5 in myocardial infarction in mice involving microRNA-27a suppression and the following GFPT2/TGF-β/Smad2/3 axis activation.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 479