Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APBB3 antibody [N3C3] (GTX109699) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX109699 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using APBB3(GTX109699) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min