APC antibody [C3], C-term

Name: APC antibody [C3], C-term
Brand: GeneTex
SKU: GTX116009
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-APC antibody [C3], C-term used in Western Blot (WB). GTX116009

Anti-APC antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116009

Anti-APC antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116009

Cat. No. GTX116009

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX116009 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC	Assay dependent

Not tested in other applications.

Calculated MW

312 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human APC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727214

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

APC regulator of WNT signaling pathway , BTPS2 , DP2 , DP2.5 , DP3 , GS , PPP1R46

Background

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq]

Database

Gene ID: 324 APC
UniProt: P25054 APC

Research Area

DATA IMAGES

GTX116009 WB Image

Non-transfected (–) and transfected (+) unboiled HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (GTX116009) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116009 ICC/IF Image

APC antibody [C3], C-term detects APC protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: APC protein stained by APC antibody [C3], C-term (GTX116009) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX116009 IHC-P Image

APC antibody [C3], C-term detects APC protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human colon cancer.
APC stained by APC antibody [C3], C-term (GTX116009) diluted at 1:500.
Antigen Retrieval: Citrate buffer, 20 min

GTX116009 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using APC(GTX116009) antibody at 1:500 dilution.

GTX116009 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (GTX116009) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116009 IHC-P Image

APC antibody [C3], C-term detects APC protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
APC antibody [C3], C-term (GTX116009) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Elisa Degl'Innocenti et al. Front Cell Neurosci 2022; 16 : 858347 Centrin 2: A Novel Marker of Mature and Neoplastic Human Astrocytes.

Application : IHC-P

Reactivity : Human

Yamaguchi S et al. Oncotarget 2018; 9 (12) : 10818-10827 Identification and characterization of a novel adenomatous polyposis coli mutation in adult pancreatoblastoma.

Moore S et al. Neuroscience 2013; 245 : 166-78 Sex chromosome complement influences functional callosal myelination.

Application : IHC

Reactivity : Mouse

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