GeneTex
United States (US)

APC antibody [FE9]

Cat No. GTX16792

Host Mouse
Clonality Monoclonal
Clone Name FE9
Isotype IgG1
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For WB: Use at a concentration of 1 - 1.5μg/ml. Predicted molecular weight: 312 kDa. (SW480 cells, which contain a mutation at codon 1338 of APC and express atruncated APC protein of 147 kDa). Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

312 kDa. ( Note )

Positive Control

HCT116 cells for p300, SW480 cells for truncated APC (p147)

Specificity/Sensitivity

Reacts with all full-length and truncated APC proteins
PROPERTIES

Form

Liquid

Buffer

50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin with =0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Immunogen used was a synthetic peptide corresponding to the N-terminal 35 amino acid residues of APC.

Purification

Purified by chromatography over Protein G Sepharose followed by FPLC.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Apc, Wnt Signaling Pathway Regulator,Btps2,Dp2,Dp2.5,Dp3,Gs,Ppp1R46,Apc

Cellular Localization

Secreted

Background

This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

Database

Research Area