APPLICATION
Application Note
For WB: Use at a concentration of 1 - 1.5μg/ml. Predicted molecular weight: 312 kDa. (SW480 cells, which contain a mutation at codon 1338 of APC and express atruncated APC protein of 147 kDa). Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
HCT116 cells for p300, SW480 cells for truncated APC (p147)
Product Note
Reacts with all full-length and truncated APC proteins
PROPERTIES
Form
Liquid
Buffer
50mM Sodium Phosphate, 0.2% Gelatin
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Immunogen used was a synthetic peptide corresponding to the N-terminal 35 amino acid residues of APC.
Purification
Purified by chromatography over Protein G Sepharose followed by FPLC.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
APC regulator of WNT signaling pathway , BTPS2 , DP2 , DP2.5 , DP3 , GS , PPP1R46
Cellular Localization
Cytoplasm, cytoskeleton , Cytoplasm , Cell membrane
Background
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
There are currently no references for APC antibody [FE9] (GTX16792). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for APC antibody [FE9] (GTX16792). Be the first to share your experience with this product.