For WB: Use at a concentration of 1 - 1.5μg/ml. Predicted molecular weight: 312 kDa. (SW480 cells, which contain a mutation at codon 1338 of APC and express atruncated APC protein of 147 kDa). Optimal dilutions/concentrations should be determined by the researcher.
HCT116 cells for p300, SW480 cells for truncated APC (p147)
Reacts with all full-length and truncated APC proteins
50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin with =0.1% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen used was a synthetic peptide corresponding to the N-terminal 35 amino acid residues of APC.
Purified by chromatography over Protein G Sepharose followed by FPLC.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Apc, Wnt Signaling Pathway Regulator,Btps2,Dp2,Dp2.5,Dp3,Gs,Ppp1R46,Apc
This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.