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APC4 antibody

Anti-APC4 antibody used in Western Blot (WB). GTX85006
Anti-APC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX85006

Cat. No. GTX85006

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all APC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

Mouse Liver Tissue Lysate(GTX27935)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

APC4 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human APC4.The immunogen is located within amino acids 40 - 90 of APC4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

anaphase promoting complex subunit 4 , APC4

Background

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.

Database

Research Area

DATA IMAGES

Anti-APC4 antibody used in Western Blot (WB). GTX85006

GTX85006 WB Image

WB analysis of mouse liver tissue lysate using GTX85006 APC4 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-APC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX85006

GTX85006 IHC-P Image

IHC-P analysis of rat liver tissue using GTX85006 APC4 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399