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APC5 antibody

Anti-APC5 antibody used in Western Blot (WB). GTX17060
Anti-APC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX17060
Anti-APC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX17060

Cat. No. GTX17060

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all APC5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

Human Kidney Tissue Lysate(GTX29995)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

APC5 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC5.The immunogen is located within amino acids 480 - 530 of APC5.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

anaphase promoting complex subunit 5 , APC5

Background

This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]

Database

Research Area

DATA IMAGES

Anti-APC5 antibody used in Western Blot (WB). GTX17060

GTX17060 WB Image

WB analysis of human kidney tissue lysate using GTX17060 ANAPC5 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-APC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX17060

GTX17060 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX17060 ANAPC5 antibody.
Working concentration : 5 μg/ml

Anti-APC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX17060

GTX17060 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX17060 ANAPC5 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399