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APC5 antibody

Anti-APC5 antibody used in Immunoprecipitation (IP). GTX33008
Anti-APC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33008
Anti-APC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33008
Anti-APC5 antibody used in Western Blot (WB). GTX33008

Cat. No. GTX33008

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all APC5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

anaphase promoting complex subunit 5 , APC5

Background

This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]

Database

Research Area

DATA IMAGES

Anti-APC5 antibody used in Immunoprecipitation (IP). GTX33008

GTX33008 IP Image

IP analysis of MCF-7 cell lysate using GTX33008 APC5 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-APC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33008

GTX33008 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX33008 APC5 antibody.
Dilution : 1:100

Anti-APC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33008

GTX33008 IHC-P Image

IHC-P analysis of human kidney tissue using GTX33008 APC5 antibody.
Dilution : 1:100

Anti-APC5 antibody used in Western Blot (WB). GTX33008

GTX33008 WB Image

WB analysis of various sample lysates using GTX33008 APC5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399