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APC7 antibody

Anti-APC7 antibody used in Western Blot (WB). GTX85187
Anti-APC7 antibody used in IHC (Paraffin sections) (IHC-P). GTX85187

Cat. No. GTX85187

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all APC7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

APC7 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human APC7.The immunogen is located within amino acids 520 - 570 of APC7.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

anaphase promoting complex subunit 7 , APC7

Background

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC7 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC7 is required for proper protein ubiquitination function of APC/C and for the interaction of APC/C with various transcription coactivators.

Database

Research Area

DATA IMAGES

Anti-APC7 antibody used in Western Blot (WB). GTX85187

GTX85187 WB Image

WB analysis of HeLa cell lysate using GTX85187 APC7 antibody.
Working concentration : 1 μg/ml

Anti-APC7 antibody used in IHC (Paraffin sections) (IHC-P). GTX85187

GTX85187 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX85187 APC7 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399