APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100 - 1:2000 |
| 1:50 - 1:200 |
| 1:100 |
| 1:10 - 1:500 |
| 1:10 - 1:500 |
| 1:10 - 1:500 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Tris-Glycine, 0.15M NaCl
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified human APE1 [UniProt# P27695]
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
apurinic/apyrimidinic endodeoxyribonuclease 1 , APE , APE1 , APEN , APEX , APX , HAP1 , REF1
Cellular Localization
Nucleus,Nucleus speckle,Endoplasmic reticulum,Cytoplasm,Nucleolus
Background
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-APE1 antibody [13B8E5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20194](/templates/default/js/lightbox/images/loading.gif "Anti-APE1 antibody [13B8E5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20194")
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GTX20194 ICC/IF Image
ICC/IF analysis of MDA-MB-231 cells using GTX20194 APE1 antibody [13B8E5C2].
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GTX20194 IHC-P Image
IHC-P analysis of human breast cancer xenograft tissue using GTX20194 APE1 antibody [13B8E5C2].
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GTX20194 WB Image
WB analysis of HeLa cell lysate using GTX20194 APE1 antibody [13B8E5C2].
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GTX20194 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX20194 APE1 antibody [13B8E5C2].
Green : primary antibody
Blue : Hoechst 33258
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GTX20194 WB Image
WB analysis of human ovarian carcinoma tissue using GTX20194 APE1 antibody [13B8E5C2].
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REFERENCE
There are currently no references for APE1 antibody [13B8E5C2] (GTX20194). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for APE1 antibody [13B8E5C2] (GTX20194). Be the first to share your experience with this product.
![Anti-APE1 antibody [13B8E5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20194](/upload/website/prouct_img/small/GTX20194/GTX20194_1601_ICC-IF_w_23060620_727.webp "Anti-APE1 antibody [13B8E5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20194")
![Anti-APE1 antibody [13B8E5C2] used in IHC (Paraffin sections) (IHC-P). GTX20194](/upload/website/prouct_img/small/GTX20194/GTX20194_468_IHC-P_w_23060620_192.webp "Anti-APE1 antibody [13B8E5C2] used in IHC (Paraffin sections) (IHC-P). GTX20194")
![Anti-APE1 antibody [13B8E5C2] used in Western Blot (WB). GTX20194](/upload/website/prouct_img/small/GTX20194/GTX20194_1113_WB_w_23060620_341.webp "Anti-APE1 antibody [13B8E5C2] used in Western Blot (WB). GTX20194")
![Anti-APE1 antibody [13B8E5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20194](/upload/website/prouct_img/small/GTX20194/GTX20194_147_ICC-IF_w_23060620_572.webp "Anti-APE1 antibody [13B8E5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20194")
![Anti-APE1 antibody [13B8E5C2] used in Western Blot (WB). GTX20194](/upload/website/prouct_img/small/GTX20194/GTX20194_1114_WB_w_23060620_643.webp "Anti-APE1 antibody [13B8E5C2] used in Western Blot (WB). GTX20194")
![Anti-APE1 antibody [13B8E5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20194](/upload/website/prouct_img/normal/GTX20194/GTX20194_1601_ICC-IF_w_23060620_727.webp "Anti-APE1 antibody [13B8E5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20194")
![Anti-APE1 antibody [13B8E5C2] used in IHC (Paraffin sections) (IHC-P). GTX20194](/upload/website/prouct_img/normal/GTX20194/GTX20194_468_IHC-P_w_23060620_192.webp "Anti-APE1 antibody [13B8E5C2] used in IHC (Paraffin sections) (IHC-P). GTX20194")
![Anti-APE1 antibody [13B8E5C2] used in Western Blot (WB). GTX20194](/upload/website/prouct_img/normal/GTX20194/GTX20194_1113_WB_w_23060620_341.webp "Anti-APE1 antibody [13B8E5C2] used in Western Blot (WB). GTX20194")
![Anti-APE1 antibody [13B8E5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20194](/upload/website/prouct_img/normal/GTX20194/GTX20194_147_ICC-IF_w_23060620_572.webp "Anti-APE1 antibody [13B8E5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20194")
![Anti-APE1 antibody [13B8E5C2] used in Western Blot (WB). GTX20194](/upload/website/prouct_img/normal/GTX20194/GTX20194_1114_WB_w_23060620_643.webp "Anti-APE1 antibody [13B8E5C2] used in Western Blot (WB). GTX20194")