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APE1 antibody [2104]

Anti-APE1 antibody [2104] used in Western Blot (WB). GTX70131

Cat No. GTX70131

Host

Mouse

Clonality

Monoclonal

Clone Name

2104

Isotype

IgG1

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
IHC-P 1:5000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HepG2, HeLa, Raji whole cell lysates

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, no preservatives added

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human APE1 purified from E. coli

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Apurinic/Apyrimidinic Endodeoxyribonuclease 1 , Ape , Ape1 , Apen , Apex , Apx , Hap1 , Ref1 , Apex1

Background

Apurinic / Apyrimidinic Endonuclease (APE 1 or REF 1) is responsible for the incision of DNA at abasicsites during base excision repair. It has also been shown to stimulate the sequence-specific DNA binding activity of Fos-Jun (AP 1) heterodimers. Alterations in APE 1 levels have been described insome cancers.

Database

Research Area

DATA IMAGES

Anti-APE1 antibody [2104] used in Western Blot (WB). GTX70131

GTX70131 WB Image

GTX70131 at a 1/1000 dilution staining ~ 38 kDa APE 1 in 35mg of:

A) HepG2 whole cell lysate; B) HeLa whole cell lysate; C) Raji whole cell lysate.

REFERENCE

REVIEW

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Package List Price ($)
$ 329