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APE1 antibody

KO/KD
Anti-APE1 antibody used in Western Blot (WB). GTX107122
Anti-APE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107122
Anti-APE1 antibody used in Western Blot (WB). GTX107122
Anti-APE1 antibody used in Western Blot (WB). GTX107122

Cat. No. GTX107122

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all APE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Mouse brain , A431 , HeLa

Predict Reactivity

Rat, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human APE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730755

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apurinic/apyrimidinic endodeoxyribonuclease 1 , APE , APE1 , APEN , APEX , APX , HAP1 , REF1

Cellular Localization

Nucleus

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-APE1 antibody used in Western Blot (WB). GTX107122

GTX107122 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX107122) diluted at 1:3000.

Anti-APE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107122

GTX107122 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using APE1(GTX107122) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-APE1 antibody used in Western Blot (WB). GTX107122

GTX107122 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX107122 diluted at 1:3000

Anti-APE1 antibody used in Western Blot (WB). GTX107122

GTX107122 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX107122 diluted at 1:1000

REFERENCE

Application Reference

Szczesny B et al. Sci Rep 2016; 6 : 36125 Inhibition of hydrogen sulfide biosynthesis sensitizes lung adenocarcinoma to chemotherapeutic drugs by inhibiting mitochondrial DNA repair and suppressing cellular bioenergetics.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169