APE1 antibody

Cat. No. GTX110558
Cat. No.  GTX110558
Anti-APE1 antibody used in Western Blot (WB). GTX110558
GTX110558 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-APE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110558
GTX110558 IHC-P Image

APE1 antibody detects APE1 protein at cytoplasm, nucleus and nucleolus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
APE1 antibody (GTX110558) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-APE1 antibody used in ChIP assay (ChIP assay). GTX110558
GTX110558 ChIP assay Image

Cross-linked ChIP was performed with 293T chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-APE1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MDR1 promoter.

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Anti-APE1 antibody used in Western Blot (WB). GTX110558
GTX110558 WB Image

APE1 antibody detects APE1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted by 1:1000.

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Anti-APE1 antibody used in Western Blot (WB). GTX110558
GTX110558 WB Image

APE1 antibody detects APEX1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10% SDS-PAGE
APE1 antibody (GTX110558) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-APE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110558
GTX110558 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using APE1(GTX110558) antibody at 1:200 dilution.

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Anti-APE1 antibody used in Western Blot (WB). GTX110558
GTX110558 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-APE1 antibody used in Immunoprecipitation (IP). GTX110558
GTX110558 IP Image

APE1 antibody immunoprecipitates APE1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : 30 μg whole cell lysate/extract of APE1 protein expressing A431 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of APE1 by 3 μg of APE1 antibody (GTX110558) 10% SDS-PAGE The immunoprecipitated APE1 protein was detected by APE1 antibody (GTX110558) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

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Anti-APE1 antibody used in Western Blot (WB). GTX110558
Anti-APE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110558
Anti-APE1 antibody used in ChIP assay (ChIP assay). GTX110558
Anti-APE1 antibody used in Western Blot (WB). GTX110558
Anti-APE1 antibody used in Western Blot (WB). GTX110558
Anti-APE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110558
Anti-APE1 antibody used in Western Blot (WB). GTX110558
Anti-APE1 antibody used in Immunoprecipitation (IP). GTX110558
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P IP ChIP assay
  • Reactivity
    Human, Mouse, Rat
See all APE1 products