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APE1 antibody

Anti-APE1 antibody used in Western Blot (WB). GTX32451
Anti-APE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32451
Anti-APE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32451
Anti-APE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32451

Cat No. GTX32451

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($329)
See all APE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

apurinic/apyrimidinic endodeoxyribonuclease 1 , APE , APE1 , APEN , APEX , APX , HAP1 , REF1

Cellular Localization

Nucleus,Nucleus speckle,Endoplasmic reticulum,Cytoplasm,Nucleolus

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-APE1 antibody used in Western Blot (WB). GTX32451

GTX32451 WB Image

WB analysis of various samples using GTX32451 APE1 antibody.
Loading : 25μg per lane

Anti-APE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32451

GTX32451 ICC/IF Image

ICC/IF analysis of A549 cell using GTX32451 APE1 antibody.

Anti-APE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32451

GTX32451 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32451 APE1 antibody.
Blue : DAPI

Anti-APE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32451

GTX32451 IHC-P Image

IHC-P analysis of human liver injury tissue using GTX32451 APE1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 329