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APE1 antibody

Anti-APE1 antibody used in Western Blot (WB). GTX70130

Cat. No. GTX70130

Host

 Mouse

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
50 μl ($399)
See all APE1 products

APPLICATION

Application Note

For WB: 1:500-1:3000. Predicted molecular weight: 40 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Full length human APE1 purified from E. coli

Purification

Unpurified
From polyclonal serum

Conjugation

Unconjugated

RRID

AB_367808

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apurinic/apyrimidinic endodeoxyribonuclease 1 , APE , APE1 , APEN , APEX , APX , HAP1 , REF1

Cellular Localization

Nucleus , Nucleus speckle , Endoplasmic reticulum , Cytoplasm , Nucleolus

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-APE1 antibody used in Western Blot (WB). GTX70130

GTX70130 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX70130) diluted at 1:1000.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399