For WB: 1:500-1:3000. Predicted molecular weight: 40 kDa. Optimal dilutions/concentrations should be determined by the researcher.
293T , A431 , HeLa , HepG2
Antiserum containing no preservatives
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Full length human APE1 purified from E. coli
From polyclonal serum
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Apurinic/Apyrimidinic Endodeoxyribonuclease 1 , Ape , Ape1 , Apen , Apex , Apx , Hap1 , Ref1 , Apex1
Apurinic / Apyrimidinic Endonuclease (APE 1 or REF 1) is responsible for the incision of DNA at abasicsites during base excision repair. It has also been shown to stimulate the sequence-specific DNA binding activity of Fos-Jun (AP 1) heterodimers. Alterations in APE 1 levels have been described insome cancers.
GTX70130 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX70130) diluted at 1:1000.