GeneTex
United States (US)

APEH antibody

GTX30054_20180530_IP_18121410_248.jpg
GTX30054_20180530_WB_18121410_215.jpg

Cat No. GTX30054

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acylaminoacyl-Peptide Hydrolase,Aare,Acph,Aph,D3F15S2,D3S48E,Dnf15S2,Oph,Apeh

Background

This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX30054_20180530_IP_18121410_248.jpg

GTX30054 IP Image

IP analysis of SW480 cell lysate using GTX30054 APEH antibody.
IP reaction : 3μg antibody / 100μg cell lysate
Dilution : 1:1000

GTX30054_20180530_WB_18121410_215.jpg

GTX30054 WB Image

WB analysis of various samples using GTX30054 APEH antibody.
Dilution : 1:1000
Loading : 25μg per lane