APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.05-0.1μg/ml |
| 5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89025-PEP).
Product Note
This antibody is expected to recognize isoform 1 (NP_001071096.1) and isoform 2 (NP_057106.2).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-HVTDRSDARLQYG, from the internal region of the protein sequence according to NP_001071096.1; NP_057106.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aph-1 homolog A, gamma-secretase subunit , 6530402N02Rik , APH-1 , APH-1A , CGI-78
Cellular Localization
Endoplasmic reticulum membrane
Background
This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
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GTX89025 IHC-P Image
IHC-P analysis of human placenta using GTX89025 APH1A antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX89025 IHC-P Image
IHC-P analysis of human kidney using GTX89025 APH1A antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX89025 WB Image
WB analysis of human brain (cerebral cortex) lysate using GTX89025 APH1A antibody, Internal.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for APH1A antibody, Internal (GTX89025). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for APH1A antibody, Internal (GTX89025). Be the first to share your experience with this product.
