GeneTex
United States (US)

APH1A antibody

GTX79306_850_ICC-IF_19011519_720.jpg
GTX79306_1919_WB_19011519_757.jpg
GTX79306_849_ICC-IF_19011519_915.jpg

Cat No. GTX79306

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS, IP, ChIP assay
Reactivity Human, Mouse, Hamster
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 2 μg/mL
FACS 3-5 μg/10? cells
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

29 kDa kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.005% sodium azide

Concentration

0.69mg/ml

Antigen Species

Human

Immunogen

Synthetic peptide, KLH conjugated, corresponding to residues C (245) R R Q E D S R V M V Y S A L R I P P E D (265) of human APH-1aL/CT.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

6530402N02Rik,APH-1,APH-1A,CGI-78

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus,Golgi stack membrane

Background

This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants. [provided by RefSeq, Aug 2011]
Show More +

Research Area

DATA IMAGES
GTX79306_850_ICC-IF_19011519_720.jpg

GTX79306 ICC/IF Image

ICC/IF analysis of A549 cells using GTX79306 APH1A antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature

GTX79306_1919_WB_19011519_757.jpg

GTX79306 WB Image

WB analysis of mouse brain and kidney extracts using GTX79306 APH1A antibody.

GTX79306_849_ICC-IF_19011519_915.jpg

GTX79306 ICC/IF Image

ICC/IF analysis of human KNS-42 glioma cells using GTX79306 APH1A antibody.