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APH1A antibody

Anti-APH1A antibody used in Western Blot (WB). GTX79306
Anti-APH1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79306
Anti-APH1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79306

Cat. No. GTX79306

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP

Reactivity

 Human, Mouse, Hamster
Package
100 μg ($399)
See all APH1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 2 μg/mL
FACS 3-5 μg/10⁶ cells
IP Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.005% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, KLH conjugated, corresponding to residues C (245) R R Q E D S R V M V Y S A L R I P P E D (265) of human APH-1aL/CT.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_11177828

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aph-1 homolog A, gamma-secretase subunit , 6530402N02Rik , APH-1 , APH-1A , CGI-78

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-APH1A antibody used in Western Blot (WB). GTX79306

GTX79306 WB Image

WB analysis of mouse brain and kidney extracts using GTX79306 APH1A antibody.

Anti-APH1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79306

GTX79306 ICC/IF Image

ICC/IF analysis of human KNS-42 glioma cells using GTX79306 APH1A antibody.

Anti-APH1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79306

GTX79306 ICC/IF Image

ICC/IF analysis of A549 cells using GTX79306 APH1A antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399