APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Slide(GTX22203) , RAW 264.7 Cell Lysate(GTX27187)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
APH1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human APH1.The immunogen is located within amino acids 80 - 130 of APH1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aph-1 homolog A, gamma-secretase subunit , 6530402N02Rik , APH-1 , APH-1A , CGI-78
Cellular Localization
Endoplasmic reticulum membrane
Background
APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gammasecretase complex which cleave the amyloid precursor protein (APP) at what are known as the gammaand gammasites and can lead to the accumulation of the Amyloid beta peptide Abetacleavage product that is associated with Alzheimer’s disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gammasecretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE.
Database
Research Area
DATA IMAGES
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GTX85061 WB Image
WB analysis of RAW264.7 cell lysate using GTX85061 APH1A antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX85061 IHC-P Image
IHC-P analysis of human brain tissue using GTX85061 APH1A antibody.
Working concentration : 5 μg/ml
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REFERENCE
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REVIEW
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