GeneTex
United States (US)

APLF antibody

APLF-antibody-GTX87979-IHC-1_18121409_459.jpg
APLF-antibody-GTX87979-WB-1_18121409_572.jpg

Cat No. GTX87979

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

56 kDa. ( Note )

Specificity/Sensitivity

APLF (Ab-116) Antibody detects endogenous levels of total APLF protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human APLF.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aprataxin And Pnkp Like Factor,Apfl,C2Orf13,Palf,Xip1,Zcchh1,Aplf

Cellular Localization

Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers.

Background

Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.

Database

DATA IMAGES
APLF-antibody-GTX87979-IHC-1_18121409_459.jpg

GTX87979 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using APLF (Ab-116) Antibody. The picture on the right is treated with the synthesized peptide.

APLF-antibody-GTX87979-WB-1_18121409_572.jpg

GTX87979 WB Image

Western blot analysis of extracts from HT-29 cells, using APLF (Ab-116) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339